Configuration Error In Apache

When i created server In Apache 2.2 on Windows Server 2008, Python 3.3 and Django 1.5. It give me this error why? This is in my conf: WSGIScriptAlias / D:/Rocnikova_prace/mysite/my

Solution 1:

Ok i Know where was the problem, It was in configuration:

<Directory D:/Rocnikova_prace/mysite/mysite><Files wsgi.py>
Options FollowSymLinks
AuthType None
AllowOverride NoneOrder deny,allow
Deny fromall</Files></Directory>

But it give me new error:

[Sun Nov 10 13:07:13 2013][error][client 127.0.0.1] client denied by server configuration: D:/Rocnikova_prace/mysite/mysite/wsgi.py

Why?